ZIA CP005803-10672 (ZIA) | |||
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Title | The Effect of Smoking on Oral Microbiota | ||
Institution | NCI, Bethesda, MD | ||
Principal Investigator | Caporaso, Neil | NCI Program Director | N/A |
Cancer Activity | N/A | Division | DCEG |
Funded Amount | $34,075 | Project Dates | 00/00/0000 - 00/00/0000 |
Fiscal Year | 2017 | Project Type | Intramural |
Research Topics w/ Percent Relevance | Cancer Types w/ Percent Relevance | ||
Biochemical Epidemiology (45.0%) Cancer (100.0%) |
Buccal Cavity (15.0%) Head and Neck (30.0%) Lung (70.0%) Pharynx (15.0%) |
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Research Type | |||
Exogenous Factors in the Origin and Cause of Cancer Technology Development and/or Marker Discovery |
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Abstract | |||
"The oral microbial community (the microbiota), is centrally related to nutrition, metabolism, immunity, inflammation, and endocrine balance. Little is known about the relationship between cigarette smoking, oral microbiota and tobacco-related health outcomes. Microbiotas in the different sites of the same oral cavity vary widely. As an initial step toward understanding the role of the oral microbiota in smoking-related health outcomes, we are initiating a pilot study to evaluate the effects of cigarette smoking on the microbiotas in buccal rinse obtained from the oral cavity and across 8 different oral cavity sites/niches (saliva, swabs from tongue dorsum, hard palate, buccal mucosa, keratinized gingiva, palatine tonsils, supragingival plaque, subgingival plaque). We will seek to recruit up to 50 volunteers through the Eastman Dental General Dentistry Clinic, Rochester, NY, including 25 current smokers with > 5 years of smoking history and 25 never smokers as a comparison group.In each subject, the buccal cell (mouthwash) collection will be collected according to the PLCO buccal cell collection protocol. In addition, collections across 8 different oral cavity sites will be done using a protocol developed for the Human Microbiome Project. These 9 collections will be used for high throughput sequencing of 16S rRNA microbial genes. The findings will provide information about the effect of smoking on microbial taxa composition and relative abundance at each collection site. " |